کد مقاله کد نشریه سال انتشار مقاله انگلیسی ترجمه فارسی نسخه تمام متن
7952041 1513706 2018 7 صفحه PDF سفارش دهید دانلود رایگان
عنوان انگلیسی مقاله ISI
One-shot method for purification of multiple natural amelogenin isoforms
ترجمه فارسی عنوان
یک روش شستشو برای تصفیه ایزوفرم های مختلف آملیوژنین طبیعی
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موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد شیمی مواد
چکیده انگلیسی
Amelogenin isoforms constitute the predominant component in the enamel matrix and each amelogenin isoform executes unique role in the enamel biomineralization process. Enamel matrix derivative enriching amelogenin isoforms have also bioactive property for tissue regeneration. Despite the development of recombinant protein technology that has greatly forwarded the understanding of amelogenin properties, substantial evidences have revealed biochemical and functional difference between natural amelogenins and their recombinant form. To facilitate the study of enamel formation mechanism, more facile methodology to purify multiple natural amelogenin isoforms is pursued. Here we developed an effective one-shot method via reverse phase high-performance liquid chromatography (RP-HPLC) to purify various amelogenin isoforms from pig-derived amelogenin complex. A thorough process of chromatographic condition establishment including sample analysis on analytical scale and chromatographic condition design on preparative scale was described. Three representative amelogenin isoforms (TRAP, P148, P173) were isolated in one step and their purity was confirmed by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and high resolution mass spectrometry.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Materials Science & Technology - Volume 34, Issue 3, March 2018, Pages 481-487
نویسندگان
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