کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
806794 1468295 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based l-glutamate sensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Real-time monitoring of extracellular l-glutamate levels released by high-frequency stimulation at region CA1 of hippocampal slices with a glass capillary-based l-glutamate sensor
چکیده انگلیسی

Real-time monitoring of l-glutamate released by high-frequency stimulation in region CA1 of mouse hippocampal slices was performed with a glass capillary-based sensor, in combination with the recoding of excitatory postsynaptic potentials (fEPSPs). A method for extracting l-glutamate currents from the recorded ones was described and applied for determining the level of extracellular l-glutamate released by 100 Hz stimulation. Recording of an l-glutamate current with a current sampling interval of 1 Hz was found to be useful for acquiring a Faradaic current that reflects l-glutamate level released by the high-frequency stimulation of 7 trains, each 20 stimuli at 100 Hz and inter-train interval of 3 s. The l-glutamate level was obtained as 15 ± 6 μM (n = 8) for the persistent enhancement of fEPSPs, i.e., the induction of long-term potentiation (LTP), and 3 ± 1 μM (n = 5) for the case of no LTP induction. Based on these observations, the level of the extracellular l-glutamate was shown to play a crucial role in the induction of LTP.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensing and Bio-Sensing Research - Volume 2, December 2014, Pages 31–37
نویسندگان
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