کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8094 577 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthetic multicellular cell-to-cell communication in inkjet printed bacterial cell systems
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Synthetic multicellular cell-to-cell communication in inkjet printed bacterial cell systems
چکیده انگلیسی

We utilized a commercially available materials printer to investigate synthetic multicellular cell-to-cell communication because inkjet printing technology makes it easy to print spatiotemporal patterns of soluble biomolecules and live cells. Since cells are genetically programmed to communicate with one another via synthetic biology, cell signaling molecules secreted by one cell microcolony can induce two neighboring cell microcolonies to respond by expressing or stopping the expression of fluorescent protein genes. In this work, we not only characterize the printing parameters such as the initial seeding numbers, spacing distances, microcolony sizes, printing timings, and printed patterns of cells but also demonstrate that the use of the proposed printing technology can provide a useful means for many synthetic biologists to simplify and speed up the investigation of cell-to-cell communication between synthetic bacterial cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 32, Issue 10, April 2011, Pages 2500–2507
نویسندگان
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