کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8274200 | 1535097 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Thiopental sodium preserves the responsiveness to glutamate but not acetylcholine in rat primary cultured neurons exposed to hypoxia
ترجمه فارسی عنوان
سدیم تیوپنتال، پاسخ به گلوتامات را حفظ می کند، اما استیل کولین را در نورون های کشت شده در موش صحرایی در معرض هیپوکسی
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کلمات کلیدی
Fmax5-FUDMEME17CCDGFAPNBSFCSAMPAHSD5-fluoro-2′-deoxyuridine - 5-fluoro-2'-deoxyuridineCa2+ - Ca2 +Dulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbecco[Ca2+]i - [Ca2 +] iACh - آهAcetylcholine - استیل کولینα-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid - اسید α-آمینو 3-هیدروکسی-5-متیل-4-ایزوکسول پپونیکgamma-aminobutyric acid - اسید گاما آمینو بوتیریکl-glutamate - ال گلوتاماتanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceDIV - دیوdays in vitro - روز in vitrohorse serum - سرب اسبfetal calf serum - سرم گوساله جنینGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایال calcium - کلسیمIntracellular calcium - کلسیم داخل سلولیGABA - گاباGlu - گلو
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
چکیده انگلیسی
Although many in vitro studies demonstrated that thiopental sodium (TPS) is a promising neuroprotective agent, clinical attempts to use TPS showed mainly unsatisfactory results. We investigated the neuroprotective effects of TPS against hypoxic insults (HI), and the responses of the neurons to l-glutamate and acetylcholine application. Neurons prepared from E17 Wistar rats were used after 2 weeks in culture. The neurons were exposed to 12-h HI with or without TPS. HI-induced neurotoxicity was evaluated morphologically. Moreover, we investigated the dynamics of the free intracellular calcium ([Ca2+]i) in the surviving neurons after HI with or without TPS pretreatment following the application of neurotransmitters. TPS was neuroprotective against HI according to the morphological examinations (0.73 ± 0.06 vs. 0.52 ± 0.07, P = 0.04). While the response to l-glutamate was maintained (0.89 ± 0.08 vs. 1.02 ± 0.09, P = 0.60), the [Ca2+]i response to acetylcholine was notably impaired (0.59 ± 0.02 vs. 0.94 ± 0.04, P < 0.01). Though TPS to cortical cultures was neuroprotective against HI morphologically, the [Ca2+]i response not to l-glutamate but to acetylcholine was impaired. This may partially explain the inconsistent results regarding the neuroprotective effects of TPS between experimental studies and clinical settings.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Neurological Sciences - Volume 365, 15 June 2016, Pages 126-131
Journal: Journal of the Neurological Sciences - Volume 365, 15 June 2016, Pages 126-131
نویسندگان
Tomotaka Morita, Satoshi Shibuta, Jun Kosaka, Yuji Fujino,