کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8287565 | 1535857 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In situ analysis of gelatinolytic activity in human dentin
ترجمه فارسی عنوان
تجزیه و تحلیل موضعی فعالیت ژلاتینولیتیک در دنتین انسان
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کلمات کلیدی
زیموگرافی در محل، متالوپروتئیناز ماتریکس، ایمونوهیستوشیمی، آنزیم ها، دندان،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Matrix metalloproteinases (MMPs) such as gelatinases are differentially expressed in human tissues. These enzymes cleave specific substrates involved in cell signaling, tissue development and remodeling and tissue breakdown. Recent evidences show that gelatinases are crucial for normal dentin development and their activity is maintained throughout the entire tooth function in the oral cavity. Due to the lack of information about the exact location and activity of gelatinases in mature human dentin, the present study was designed to examine gelatinolytic levels in sound dentin. In situ zymography using confocal microscopy was performed on both mineralized and demineralized dentin samples. Sites presenting gelatinase activity were identified throughout the entire biological tissue pursuing different gelatinolytic levels for distinct areas: predentin and dentinal tubule regions presented higher gelatinolytic activity compared to intertubular dentin. Dentin regions with higher gelatinolytic activity immunohistochemically were partially correlated with MMP-2 expression. The maintenance of gelatinolytic activity in mature dentin may have biological implications related to biomineralization of predentin and tubular/peritubular dentinal regions, as well as regulation of defensive mechanisms of the dentin-pulp complex.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 120, Issue 2, February 2018, Pages 136-141
Journal: Acta Histochemica - Volume 120, Issue 2, February 2018, Pages 136-141
نویسندگان
Thiago Henrique Scarabello Stape, Leo Tjäderhane, Arzu Tezvergil-Mutluay, Wagner Gomes Da Silva, Alan Roger dos Santos Silva, Wander José da Silva, Marcelo Rocha Marques,