کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8298362 | 1536893 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro interactions of histones and α-crystallin
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کلمات کلیدی
PBSITCGPCCataract - آب مرواریدComplex formation - تشکیل پیچیدهRALS - رالزMALDI-TOF MS - مالدی توف MSPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHigh salt - نمک بالاHistone - هیستونIsothermal titration calorimetry - کالری سنجی تیتاسیون ایزوترمالGel permeation chromatography - کروماتوگرافی ژل تراوشیcrystallin - کریستالین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
The aggregation of crystallins in lenses is associated with cataract formation. We previously reported that mutant crystallins are associated with an increased abundance of histones in knock-in and knockout mouse models. However, very little is known about the specific interactions between lens crystallins and histones. Here, we performed in vitro analyses to determine whether α-crystallin interacts with histones directly. Isothermal titration calorimetry revealed a strong histone-α-crystallin binding with a Kd of 4â¯Ãâ¯10â7 M, and the thermodynamic parameters suggested that the interaction was both entropy and enthalpy driven. Size-exclusion chromatography further showed that histone-α-crystallin complexes are water soluble but become water insoluble as the concentration of histones is increased. Right-angle light scattering measurements of the water-soluble fractions of histone-α-crystallin mixtures showed a decrease in the oligomeric molecular weight of α-crystallin, indicating that histones alter the oligomerization of α-crystallin. Taken together, these findings reveal for the first time that histones interact with and affect the solubility and aggregation of α-crystallin, indicating that the interaction between α-crystallin and histones in the lens is functionally important.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 15, September 2018, Pages 7-12
Journal: Biochemistry and Biophysics Reports - Volume 15, September 2018, Pages 7-12
نویسندگان
Paul D. Hamilton, Usha P. Andley,