کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8298423 | 1536894 | 2018 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DOCK9 induces membrane ruffles and Rac1 activity in cancer HeLa epithelial cells
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کلمات کلیدی
RufflesGSTp21-binding domainDOCK10DOCKRac1PBDPAK1DOXTTAGEFp21-activated kinase 1 - p21-فعال کیناز 1Tetracycline transactivator - تتراسیکلین ترانسکتیویتورdoxycycline - داکسی سایکلینactin cytoskeleton - سی تی اسکن آکتینGuanine-nucleotide exchange factor - فاکتور تبادل گوانین-نوکلئوتیدیwild type - نوع وحشیhemagglutinin - هماگلوتینینglutathione S-transferase - گلوتاتیون S-ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dedicator-of-cytokinesis (DOCK) proteins are a family of guanine-nucleotide exchange factors (GEF) for Rho GTPases. The DOCK-D homology subfamily comprises DOCK9, DOCK10, and DOCK11. DOCK9 and DOCK11 are GEFs for Cdc42 and induce filopodia, while DOCK10 is a dual GEF for Cdc42 and Rac1 and induces filopodia and ruffles. We provide data showing that DOCK9, the only one of the DOCK-D members that is not considered hematopoietic, is nevertheless expressed at high levels in T lymphocytes, as do DOCK10 and DOCK11, although unlike these, it is not expressed in B lymphocytes. To investigate DOCK9 function, we have created a stable HeLa clone with inducible expression of HA-DOCK9. Induction of expression of HA-DOCK9 produced loss of elongation and polygonal shape of HeLa cells. Regarding membrane protrusions, HA-DOCK9 prominently induced filopodia, but also an increase of membrane ruffles. The latter was consistent with an increase in the levels of activation of Rac1, suggesting that DOCK9 carries a secondary ability to induce ruffles through activation of Rac1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemistry and Biophysics Reports - Volume 14, July 2018, Pages 178-181
Journal: Biochemistry and Biophysics Reports - Volume 14, July 2018, Pages 178-181
نویسندگان
Natalia Ruiz-Lafuente, Alfredo Minguela, Antonio Parrado,