کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8336122 | 1540432 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Baseline striatal and nigral interneuronal protein levels in two distinct mice strains differ in accordance with their MPTP susceptibility
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کلمات کلیدی
PFAsubstantia nigra pars reticulateCAL-B1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridineinterneuronsTBSTMSNNurr1MPTPGADDABPBSBSA - BSAPBS-Tx - PBS-TXSNpc - SNPCSNpr - SNPRγ-amino butyric acid - γ-آمینو اسید بوتیریکHydrogen peroxide - آب اکسیژنهbovine serum albumin - آلبومین سرم گاوStriatum - استریاتومImmunohistochemistry - ایمونوهیستوشیمیIHC - ایمونوهیستوشیمیParkinson’s disease - بیماری پارکینسونParkinson's disease - بیماری پارکینسونsubstantia nigra pars compacta - توده سیاه پارس متراکمtyrosine hydroxylase - تیروزین هیدروکسیلازPhosphate buffered saline - فسفات بافر شورH2O2 - هیدروژن پراکسیدparaformaldehyde - پارافرمالدهیدParvalbumin - پاروالبومینCalretinin - کالورتینینcalbindin - کلبیندینGABA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Epidemiological studies reveal an ethnicity-based bias in prevalence of Parkinson's disease (PD), deriving from the differences that exist between Caucasians and African or Asian populations. Experimental mice models provide a scope to analyse the cellular mechanisms of differential susceptibility to PD. C57BL/6J mice, for instance, are more susceptible to MPTP-induced Parkinsonism whereas CD-1 mice are resistant. In PD-pathogenesis, interneuronal contribution is also likely, although they comprise only 5-10% of the striatal cells. The interneurons harbour calcium binding proteins, like calretinin (Cal-R) and parvalbumin (PV), which are crucial in Ca2+ homeostasis for preventing calcium-induced excitotoxicity. GAD-67-immunoreactive interneurons are the other prominent set of GABAergic interneurons. In PD, dopamine loss up-regulates GAD-67 expression in striatal projection neurons and other basal ganglia circuit. We studied the possible contribution of interneurons in determining variable susceptibility by assessing the expression of calretinin, PV and GAD-67 in both striatum and substantia nigra pars compacta (SNpc) in two distinct mice strains, i.e. C57BL/6J and CD-1 under normal conditions, using unbiased stereology for quantification of immunoreactive cells and immunoblotting. The vulnerable C57BL/6J had lesser basal parvalbumin expression in both nigra and striatum whereas the calretinin levels were low only in the striatum. GAD-67 expression showed no perceptible differences in the striatum or SNpc of either of the strains. Differential expression of calcium buffering/binding proteins under normal physiological condition proffers a role for interneurons in the differential susceptibility to PD. Thus, even the baseline susceptibility indices i.e. without using the neurotoxin; can provide vital mechanistic insights into PD pathogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chemical Neuroanatomy - Volume 91, September 2018, Pages 46-54
Journal: Journal of Chemical Neuroanatomy - Volume 91, September 2018, Pages 46-54
نویسندگان
Bidisha Bhaduri, P.L. Abhilash, Phalguni Anand Alladi,