کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8348176 | 1541716 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Secretion of urocortin I by human glioblastoma cell lines, possibly via the constitutive pathway
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
DMEMUCNCRF-R2CRF-R1diaminobenzidine tetrahydrochloridehNSCATCCCRFFBSDABDMSO - DMSODulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoUrocortin - اوروکورتینTem - این استConstitutive secretion - ترشح مؤثرCNS - دستگاه عصبی مرکزیDimethylsulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوHuman neural stem cell - سلول های بنیادی عصبی انسانcentral nervous system - سیستم عصبی مرکزیcorticotropin-releasing factor - عامل تخریب کورتیکوتروپینAmerican Type Culture Collection - مجموعه فرهنگی نوع آمریکاییTransmission electron microscope - میکروسکوپ الکترونی انتقالFluorescent protein - پروتئین فلورسنت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Corticotropin-releasing factor (CRF) and its family of peptides, i.e., urocortins (UCNs), play a critical role in systemic and peripheral stress-response systems and are widely expressed not only in normal tissues but also in various types of cancer cells. Given limited understanding of the mechanism of UCN I secretion, we investigated the UCN I secretory pathway in human neural stem cells (HNSCs) and in two glioblastoma cell lines, e.g., A172 and U-138 MG. Immunoreactivities for CRF receptors were detected in A172 glioblastoma cells, but not in HNSCs or U-138 glioblastoma cells, while UCN I immunoreactivity was detected in A172 and U-138 MG glioblastoma cell lines by both light field and electron microscopy. Interestingly, electron microscopy revealed UCN I immunoreactivtiy in vesicle-like structures in the plasma membrane of the glioblastoma cells. Tracking of a hybrid fluorescent protein containing a UCN I signal peptide expressed in A172 human glioblastoma cells revealed that fluorescence in secretory granules could be decreased by cycloheximide (100 μg/ml), indicating that the forward transport of secretory granules containing fluorescent protein was not altered by the inhibition of protein synthesis by cycloheximide. Retrograde transport and the fusion of fluorescent granules in A172 human glioblastoma cells was induced by brefeldin A (10 μg/ml), indicating that UCN I secretory granules may be transported via the constitutive pathway. Based on these results, it appears that UCN I is secreted from human glioblastoma cells by exocytosis through constitutive secretory granules, indicating that transcription of UCN I mRNA may be correlated to secretion of UCN I protein.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 63, January 2015, Pages 63-70
Journal: Peptides - Volume 63, January 2015, Pages 63-70
نویسندگان
Keiichi Ikeda, Kouki Fujioka, Toshiaki Tachibana, Seung U. Kim, Katsuyoshi Tojo, Yoshinobu Manome,