کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8348571 | 1541732 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
C-type natriuretic peptide transcriptomic profiling increases in human leukocytes of patients with chronic heart failure as a function of clinical severity
ترجمه فارسی عنوان
پروفیل ترانسکتکتومی پپتید سدیم نوع نیتروئیدیک در لکوسیت های انسانی بیماران مبتلا به نارسایی مزمن قلبی به عنوان تابع شدت بالینی
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
The aim of this study was to evaluate the transcriptomic profiling of C-type natriuretic peptide (CNP) and of its specific receptor, NPR-B in human leukocytes of heart failure (HF) patients as a function of clinical severity, assessing the possible changes with respect to healthy subjects (C). mRNA expression was evaluated by Real-Time PCR and total RNA was extracted from leukocytes of C (n = 8) and of HF patients (NYHA I-II, n = 7; NYHA III-IV, n = 13) with PAXgene Blood RNA Kit. Significantly higher levels of CNP mRNA expression were found in HF patients as a function of clinical severity (C = 0.23 ± 0.058, NYHA I-II = 0.47 ± 0.18, NYHA III-IV = 2.58 ± 0.71, p = 0.005 C vs NYHA III-IV, p = 0.017 NYHA I-II vs NYHA III-IV) and NPR-B transcript levels resulted down-regulated in HF patients with higher NYHA class (C = 2.2 ± 0.61, NYHA I-II = 2.76 ± 0.46, NYHA III-IV = 0.29 ± 0.13, p = 0.001 C vs NYHA III-IV, p < 0.0001 NYHA I-II vs NYHA III-IV). A significant negative correlation between CNP and NPR-B mRNA expression (r = 0.5, p = 0.03) was also observed. These results suggest a co-regulation of NPR-B and CNP expression supporting the relevance of this receptor in human disease characterized by a marked inflammatory/immune component and suggesting the possibility of manipulating inflammation via pharmacological agents selective for this receptor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 47, September 2013, Pages 110-114
Journal: Peptides - Volume 47, September 2013, Pages 110-114
نویسندگان
M. Cabiati, L. Sabatino, R. Caruso, A. Verde, C. Caselli, T. Prescimone, D. Giannessi, S. Del Ry,