The differential extraction and immunoluminometric assay of Urotensin II and Urotensin-related peptide in heart failure

► We differentially extracted Urotensin II and Urotensin related peptide from plasma. ► We used a novel immunoluminometric assay to measure the peptides. ► We measured these peptides in controls and acute heart failure patients. ► Both peptides were elevated in heart failure patients compared to controls. ► This further supports a role of the Urotensin system in heart failure.