کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8362816 | 1542564 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of temperature, water content, pH and soil sterilization on the degradation of CP4-EPSPS protein released from herbicide-tolerant corn leaves in the soil
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش خاک شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Determining the influence of soil environmental factors on the degradation of CP4-EPSPS protein from herbicide-tolerant (HT) corn residue is crucial for assessing the ecological risks of commercialized transgenic crops. We investigated the effects of soil temperature (15â¯Â°C, 25â¯Â°C, 35â¯Â°C), soil water content (20%, 33%, 50%), pH (5.0, 7.0, 9.0) and soil treatment (not sterilized, sterilized) on the degradation of CP4-EPSPS protein released from the leaves of the HT corn variety CC-2. An exponential model (first order) was fitted to the degradation dynamics of CP4-EPSPS protein and used to estimate the time required for 50% degradation (DT50) and for 90% degradation (DT90). The results showed that CP4-EPSPS protein released from CC-2 leaves presented similar degradation features in all treatments: a sharp decrease in the early stage followed by a slow decline in the middle and late stages was observed. In the late stage (more than 120â¯d after the experiment started), CP4-EPSPS protein released from CC-2 leaves was not detected in the soil using enzyme-linked immunosorbent assay (ELISA) tests. The DT50 values of CP4-EPSPS protein released from CC-2 leaves ranged from 0.92â¯d to 11.32â¯d, and the DT90 values ranged from 3.11â¯d to 36.59â¯d. The results suggest that soil temperature and soil sterilization significantly affected the degradation of CP4-EPSPS protein. Faster degradation rates were observed at higher temperature under non-sterilized conditions, but soil water content and pH did not clearly affect the degradation of CP4-EPSPS protein. These findings suggest that under appropriate soil temperature conditions, CP4-EPSPS protein from HT corn residue will not persist or accumulate in the soil.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 120, May 2018, Pages 165-170
Journal: Soil Biology and Biochemistry - Volume 120, May 2018, Pages 165-170
نویسندگان
Xing Zeng, Kang Wang, Chenchen Feng, Xinyuan Song, Fengkun Qi, Lin Zhang, Ling Dong, Yu Zhou, Xianjun Liu, Zhenhua Wang, Hong Di,