کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8364538 | 1542608 | 2014 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
High-throughput sequencing shows inconsistent results with a microscope-based analysis of the soil prokaryotic community
ترجمه فارسی عنوان
توالی با شدت بالا نتیجه گیری های متناقض با یک تجزیه و تحلیل میکروسکوپ مبتنی بر جامعه پروکریوت خاک نشان می دهد
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش خاک شناسی
چکیده انگلیسی
In the present study, we perform the first direct analysis on how the composition of the prokaryotic soil community differs depending on whether high-throughput sequencing or fluorescent in situ hybridization (FISH) coupled with catalyzed reporter deposition (CARD) is used. Soil samples were collected along short (<3 m) tundra vegetation gradients from Northern Sweden. Relative abundances of Acidobacteria and Bacteroidetes estimated by the high-throughput sequencing were higher than those estimated by CARD-FISH, while relative abundances of Archaea and α-Proteobacteria estimated by high-throughput sequencing were lower than those estimated by CARD-FISH. The results indicated that the high-throughput sequencing overestimates/underestimates the relative abundance of some microbial taxa if we assume that CARD-FISH can provide potentially more quantitative data. Great caution should be taken when interpreting data generated by molecular technologies (both of high-throughput sequencing and CARD-FISH), and supports by multiple approaches are necessary to make a robust conclusion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 76, September 2014, Pages 53-56
Journal: Soil Biology and Biochemistry - Volume 76, September 2014, Pages 53-56
نویسندگان
Masayuki Ushio, Kobayashi Makoto, Jonatan Klaminder, Hiroyuki Takasu, Shin-ichi Nakano,