کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8371 | 588 | 2010 | 9 صفحه PDF | دانلود رایگان |
In the present study, polymer monofilaments were embedded in fibrin seeded with human umbilical vein endothelial cells (HUVEC) to guide HUVEC attachment and migration in order to form oriented vessel-like structures between adjacent monofilaments. Histology and fluorescent fibrin experiments confirmed that microvessel-like structures, which were developing between polymer monofilaments embedded in fibrin, contained a lumen. The effect of human fibroblasts and growth factors (VEGF and bFGF) over the microvessel formation process was tested. The effects of VEGF and bFGF were dose-dependent. The effect of VEGF was optimum at the lower concentration tested (2 ng/mL), while that of bFGF was optimum at the higher tested concentration (20 ng/mL). Furthermore, the use of fibroblasts significantly improved the maturation of the microvessels compared to control and to samples cultured with VEGF and bFGF.
Journal: Biomaterials - Volume 31, Issue 19, July 2010, Pages 5091–5099