کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8383978 | 1543461 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Calcium ions inhibit reduction of heme a in bovine cytochrome c oxidase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The effect of Ca2+ on the rate of heme a reduction by dithionite and hexaammineruthenium (RuAm) was studied in the cyanide-complexed bovine cytochrome oxidase (CcO). The rate of heme a reduction is proportional to RuAm concentration below 300 μM with kv of 0.53 Ã 106 Mâ1sâ1. Ca2+ inhibits the rate of heme a reduction by dithionite by â¼25%. As the reaction speeds up with increased concentrations of RuAm, the inhibition by Ca2+ disappears. The inhibition of heme a reduction may contribute to recently described partial inhibition of CcO by Ca2+ in the enzymatic assays. The inhibitory effect of Ca2+ on heme a reduction indicates that ET through heme a may be coupled to proton movement in the exit part of the proton channel H.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 589, Issue 24, Part B, 21 December 2015, Pages 3853-3858
Journal: FEBS Letters - Volume 589, Issue 24, Part B, 21 December 2015, Pages 3853-3858
نویسندگان
Artem V. Dyuba, Tatiana Vygodina, Natalia Azarkina, Alexander A. Konstantinov,