کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8384082 1543463 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Importance of C-terminal flexible region of 4E-binding protein in binding with eukaryotic initiation factor 4E
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Importance of C-terminal flexible region of 4E-binding protein in binding with eukaryotic initiation factor 4E
چکیده انگلیسی
Although the α-helical Y(X)4Lϕ containing region of eIF4E-binding protein (4EBP) is the major binding region with eukaryotic initiation factor 4E (eIF4E), the roles of its N- and C-terminal regions in the binding are hardly known. To clarify the roles of these flexible regions in the interaction, the binding features of the sequentially N-, C-, or both-terminal-residue-deleted 4EBP2 mutants were investigated by surface plasmon resonance (SPR) analysis. It was shown that the C-terminal His74-Glu89 sequence has an auxiliary, but indispensable, function in stabilizing the binding to eIF4E. The possible interaction with eIF4E was estimated by molecular dynamics simulation. This is the first report on the importance of the C-terminal flexible region in the eIF4E-binding regulation of 4EBP.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 582, Issues 23–24, 15 October 2008, Pages 3439-3444
نویسندگان
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