کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8384134 | 1543463 | 2008 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Nuclear matrix contains novel WD-repeat and disordered-region-rich proteins
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
To find novel proteins predicted to participate in the formation of nuclear bodies, nuclear speckles, and nuclear macro-protein complexes, we applied proteome analysis to a HeLa cell nuclear matrix fraction. Proteins in the fraction were separated by SDS-PAGE, digested with trypsin, and analyzed by nanoflow liquid chromatography-iontrap-tandem mass spectrometry. Three hundred and thirty three proteins including 39 novel ones were identified. Seven WD-repeat proteins and 16 disordered region-rich proteins, which act frequently as scaffolding proteins for macro-protein complexes, were found amongst the novel proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 582, Issues 23â24, 15 October 2008, Pages 3515-3519
Journal: FEBS Letters - Volume 582, Issues 23â24, 15 October 2008, Pages 3515-3519
نویسندگان
Kohei Ishii, Yasuhiro Hirano, Nanase Araki, Toshiyuki Oda, Masahiro Kumeta, Kunio Takeyasu, Kazuhiro Furukawa, Tsuneyoshi Horigome,