کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8385051 | 1543668 | 2017 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Temporal regulation of ÏB by partner-switching mechanism at a distinct growth stage in Bacillus cereus
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
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چکیده انگلیسی
The alternative transcription factor ÏB in Bacillus cereus governs the transcription of a number of genes that confer protection against general stress. This transcription factor is regulated by protein-protein interactions among RsbV, RsbW, ÏB, RsbY, RsbM and RsbK, all encoded in the sigB cluster. Among these regulatory proteins, RsbV, RsbW and ÏB comprise a partner-switching mechanism. Under normal conditions, ÏB remains inactive by associating with anti-sigma factor RsbW, which prevents ÏB from binding to the core RNA polymerase. During environmental stress, RsbK activates RsbY to hydrolyze phosphorylated RsbV, and the dephosphorylated RsbV then sequesters RsbW to liberate ÏB from RsbW. Although the ÏB partner-switching module is thought to be the core mechanism for ÏB regulation, the actual protein-protein interactions among these three proteins in the cell remain to be investigated. In the current study, we show that RsbW and RsbV form a long-lived complex under transient stress treatment, resulting in high persistent expression of RsbV, RsbW and ÏB from mid-log phase to stationary phase. Full sequestration of RsbW by excess RsbV and increased RsbW:RsbV complex stability afforded by cellular ADP contribute to the prolonged activation of ÏB. Interestingly, the high expression levels of RsbV, RsbW and ÏB were dramatically decreased beginning from the transition stage to the stationary phase. Thus, protein interactions among ÏB partner-switching components are required for the continued induction of ÏB during environmental stress in the log phase and significant down-regulation of ÏB is observed in the stationary phase. Our data show that ÏB is temporally regulated in B. cereus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Medical Microbiology - Volume 307, Issue 8, December 2017, Pages 521-532
Journal: International Journal of Medical Microbiology - Volume 307, Issue 8, December 2017, Pages 521-532
نویسندگان
Jung-Chi Chen, Chuan-Fu Chang, Duen-Wei Hsu, Jwu-Ching Shu, Hong-Yi Chen, Chien-Yen Chen, Chi-Yu Lu, Chien-Cheng Chen,