کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8397715 | 1544165 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cytotoxic activity of extracts from Hypochaeris radicata
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کلمات کلیدی
HBSSHypochaeris radicataMSCCPLLSGCPBSDMEMDMSO - DMSOHorse - اسبDulbecco's minimal essential medium - حداقل کالای ضروری DulbeccoDimethyl sulfoxide - دیمتیل سولفواکسیدlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Hank's buffered salt solution - محلول نمک بافر هانکPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNeurotoxin - نوروتوکسینPoly-l-lysine - پلی-لیزینperipheral neuropathy - پلینوریت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cytotoxic activity of extracts from Hypochaeris radicata Cytotoxic activity of extracts from Hypochaeris radicata](/preview/png/8397715.png)
چکیده انگلیسی
Pasture-associated stringhalt is an acquired equine disease characterized by peripheral neuropathy and hyperflexion of the pelvic limbs. The disease occurs most commonly during periods of drought in horses grazing pastures heavily contaminated by Hypochaeris radicata. We hypothesized that stringhalt is caused by neurotoxins elaborated by H. radicata in response to the stress of drought conditions. Supernates were collected from H. radicata that were stressed (or not) by immersion in copper chloride solution, then extracted with ethyl acetate and dried. Dilutions of extracts from stressed (SE) and control, unstressed (UE) plants were incubated with myelinating spinal cord cultures (MSCC) established from fetal Swiss mice, and with spinal ganglion cultures (SGC) and dermal fibroblast cultures derived from neonatal mouse tissues. Cytotoxicity in culture monolayers was evaluated both morphologically by microscopy and by release of lactate dehydrogenase activity into culture supernates. Three different SGC preparations were exposed to a single H. radicata extract and single preparations of fibroblasts and MSCC were exposed to three different extracts. Repin, a plant-derived sesquiterpene lactone neurotoxin, was included as a positive control. Significant dose-dependent cytotoxicity was seen within 24 h in all three culture types when incubated with SE or repin. Complete morphologic destruction of culture monolayers was induced by the highest concentrations tested of SE (100 μg/mL) and repin (30 μg/mL). Cytotoxic effect of SE was significantly greater than that of UE for all three cell types and was not due to copper contamination of the extract. This study has identified a cytotoxic activity in leaf exudates of H. radicata that was upregulated by the model stressor, copper chloride.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicon - Volume 70, August 2013, Pages 194-203
Journal: Toxicon - Volume 70, August 2013, Pages 194-203
نویسندگان
R.J. MacKay, S. Wyer, A. Gilmour, K. Kongara, D.R. Harding, S. Clark, I.G. Mayhew, C.E. Thomson,