کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8403765 | 1544682 | 2018 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Is boar sperm freezability more intrinsically linked to spermatozoa than to the surrounding seminal plasma?
ترجمه فارسی عنوان
آیا آزاد شدن اسپرم قارچ به طور ذاتی به اسپرماتوزا نسبت به پلاسما مایع منی مرتبط است؟
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کلمات کلیدی
گندزدایی اسپرم، پلاسمای سمی، قسمت های انزال مشخصات پروتئین اسپرم، گراز،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
This study aimed to elucidate the effect of seminal plasma (SP) from post-SRF on boar sperm freezability and, in addition, to determine the relevance of sperm itself to sustain cryopreservation, regardless of the SP surrounding them. Twelve ejaculates from three boars were manually collected in fractions/portions, P1: the first 10â¯mL of the SRF, P2: the rest of the SRF and the post-SRF. Immediately, samples were centrifuged to separate spermatozoa from the surrounding SP. Spermatozoa from P1 and P2â¯were then incubated with its own SP or that from post-SRF, diluted in BTS (1:1, v/v) at 17â¯Â°C overnight before being frozen in 0.5â¯mL straws using a standard protocol. Sperm motility (total and progressive) deteriorated (Pâ¯<â¯0.05) when P1- or P2-sperm when incubated overnight in SP from post-SRF, while sperm viability differed between P1 and P2 (Pâ¯<â¯0.05) regardless of the SP they were incubated in. Post-thaw sperm quality and functionality differed between P1 and P2, regardless of the SP used for overnight pre-freezing incubation. Post-thaw motility (Pâ¯<â¯0.05) and viability (Pâ¯<â¯0.01), as well as plasma membrane fluidity (Pâ¯<â¯0.05) or lipid peroxidation values (Pâ¯<â¯0.01) were best in P1 sperm compared to those of P2. The protein profile of sperm from P1 and P2, analyzed by 2D-PAGE, showed qualitative differences, which suggest that sperm rather than SP would explain differences in sperm freezability between ejaculate fractions/portions. Use of P1 fraction spermatozoa seems thus optimal for cryopreservation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 195, August 2018, Pages 30-37
Journal: Animal Reproduction Science - Volume 195, August 2018, Pages 30-37
نویسندگان
Junwei Li, Jordi Roca, Cristina Pérez-Patiño, Isabel Barranco, Emilio A. Martinez, Heriberto Rodriguez-Martinez, Inmaculada Parrilla,