Embryonic development, luteal size and blood flow area, and concentrations of PGF2α metabolite in dairy cows fed a diet enriched in polysaturated or polyunsaturated fatty acid

The objective of this study was to examine effects of sunflower (SO) and palm oil (PO) supplements in the diet on embryonic development, luteal size and blood flow area, PGF2α metabolite (PGFM), and progesterone (P4) concentrations. Prepartum cows (n = 42) were randomly assigned to one of three dietary treatments (control, 4% PO, and 4% SO supplements). Animals were fed diets individually from day 28 prepartum to day 111 postpartum. Luteal size and blood flow area were determined throughout the estrous cycle by Doppler ultrasonography. Oocytes were collected in three ovum pick-up sessions at 2 week intervals for the in vitro embryo production. Oocyte characteristics and embryonic development were not affected by dietary treatments. Cows fed 4% SO had a greater (P < 0.05) concentration of PGFM from day 15 to day 35 postpartum than those cows fed 4% PO and the control group. On day 11 of the estrous cycle (mid-luteal phase), serum P4 concentrations (6.0 ± 0.7, 5.7 ± 0.5, and 4.7 ± 0.6 ng/ml), luteal size (7.0 ± 0.2, 6.5 ± 0.2, and 5.3 ± 0.1 cm2) and luteal blood flow area (1.3 ± 0.2, 1.2 ± 0.1, and 0.9 ± 0.1 cm2) were greater (P < 0.05) in cows fed 4% SO and 4% PO than the control group, respectively. Thus, plant oil supplements in diets affected luteal size and serum P4 and PGFM concentrations, but not early embryonic development. Such changes in secretion of PGF2α and P4 indicate that plant oil supplements during pre- and postpartum may alter uterine and luteal functions.