کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8403883 | 1544685 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of immunization against bone morphogenetic protein-15 and growth differentiation factor-9 on ovarian function in mares
ترجمه فارسی عنوان
اثرات ایمن سازی در برابر پروتئین 15-مورفوژنیک استخوان و عامل تکثیر رشد 9 بر عملکرد تخمدان در موش خرما
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
چکیده انگلیسی
Currently there is no contraceptive vaccine that can cause permanent sterility in mares. This study investigates the effect of vaccination against oocyte-specific growth factors, Bone Morphogenetic Protein 15 (BMP-15) and Growth Differentiation Factor 9 (GDF-9), on ovarian function of mares. It was hypothesized that immunization against these growth factors would prevent ovulation and/or accelerate depletion of the oocyte reserve. For this study, 30 mares were randomly assigned to three groups (nâ¯=â¯10/group) and vaccinated with BMP-15 or GDF-9 peptides conjugated to KLH and adjuvant, or a control of phosphate buffered saline and adjuvant. Horses received vaccinations at weeks 0, 6, 12, and 18. Ovarian activity and estrous behavior were evaluated 3 days a week via ultrasonography and interaction with a stallion. The study was initiated on March1, 2016. Upon evaluation of ovulation rate, the GDF-9 group did not have a difference (Pâ¯=â¯0.66) in ovulation rate when compared to controls (10.8 and 10.0 ovulations, respectively), but the number of ovulations in the BMP-15 group was less (Pâ¯=â¯0.02; 4.9 ovulations). Average follicle size prior to ovulation was less (Pâ¯<â¯0.0001) in both treatment groups compared to controls. Estrous behavior was altered in both the BMP-15 and GDF-9 groups compared to controls after the second vaccination (Pâ¯=â¯0.05 and 0.03, respectively). Although further research is required to determine the continued effects of vaccination against GDF-9 on ovulation rates, these results indicate that vaccination against BMP-15 and GDF-9 could serve as a contraceptive in wild horse populations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 192, May 2018, Pages 69-77
Journal: Animal Reproduction Science - Volume 192, May 2018, Pages 69-77
نویسندگان
Kelli A. Davis, Kristin M. Klohonatz, Darcy S.O. Mora, Hannah M. Twenter, Peter E. Graham, Pablo Pinedo, Douglas C. Eckery, Jason E. Bruemmer,