کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8404029 | 1544685 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impact of cryopreservation on sterlet, Acipenser ruthenus sperm motility and proteome
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Fish sperm cryopreservation is a well-established technique allowing for artificial insemination on a commercial scale. The extent of proteome alterations in seminal plasma and sperm due to cryopreservation, however, is not known. This study was conducted to evaluate the effect of cryopreservation on motility variables of sterlet Acipenser ruthenus sperm and to detect the differences in protein profiles of fresh and cryopreserved sterlet sperm and seminal plasma. Fresh sperm had 89â¯Â±â¯3% motility and 160â¯Â±â¯14â¯Î¼m/s curvilinear velocity at 15â¯s post-activation. The motility rate of cryopreserved sperm (37â¯Â±â¯5%) was less at 15â¯s post-activation. No difference (ANOVA; Pâ¯>â¯0.05) in mean curvilinear velocity of fresh and cryopreserved sperm was detected. The protein profiles of seminal plasma and sperm were characterized using comparative proteomics to determine the influence of cryopreservation. Six altered protein spots in seminal plasma and thirteen altered spots in sperm were detected in fresh and thawed sperm. Subsequent protein characterization suggested that the proteins identified were involved in sperm metabolism, cytoskeleton, and stress response. The results broaden the understanding of the effects of cryopreservation and identify the proteins associated with cryo-injury. These data may help to determine the function of altered proteins and provide new insights into improving sperm cryopreservation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 192, May 2018, Pages 280-289
Journal: Animal Reproduction Science - Volume 192, May 2018, Pages 280-289
نویسندگان
Miaomiao Xin, Anna Shaliutina-Kolesova, Jan Sterba, Peter Konik, Sergii Boryshpolets, Marek Rodina, Ping Li, Rui Nian, Otomar Linhart,