کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8404056 | 1544689 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Studies of the cryopreservation condition of Gymnocypris przewalskii spermatozoa
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
The endemic naked carp (Gymnocypris przewalskii Kessler, 1876) plays an important role in the maintenance of the distinctive ecological system of Lake Qinghai at 3.2 km altitude on the Qinghai-Tibet Plateau in China. This study aimed to develop a cryopreservation protocol for Gymnocypris przewalskii spermatozoa. Semen was collected from mature individuals during migration and frozen using the liquid nitrogen vapor method. The influence of different cryoprotectants and three extenders on the post-thaw quality of the sperm was analyzed. The highest sperm motility rate and longest motility time after cryopreservation were achieved by combining Ringer's solution with 15% ethylene glycol (P < 0.05). The fertilization rate of this cryopreserved semen was 15.26 ± 4.54%. This study thus provides a valuable method for the cryopreservation of the sperm of this important endangered fish species.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Animal Reproduction Science - Volume 188, January 2018, Pages 13-20
Journal: Animal Reproduction Science - Volume 188, January 2018, Pages 13-20
نویسندگان
Fulei Wei, Luxian Yu, Ruihong Li, Xia Zhang, Xuehan Zhang, Ya Zhang, Yuqing Wang, Hongchao Wang, Jian Liang, Rui Ma, Hongfang Qi, Qiwei Qin, Rongqing Zhang, Shihai Zhu, Changzhong Li,