کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8443584 | 1547124 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Single domain antibody against carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) inhibits proliferation, migration, invasion and angiogenesis of pancreatic cancer cells
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کلمات کلیدی
FACSDMEMIgGMMP-9MMP-2CEACAM6DAPI4′,6-diamidino-2-phenylindole - 4 '، 6-دیامیدینو-2-فنیلینولDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده DulbeccoSingle domain antibodies - آنتی بادیهای دامنهAngiogenesis - آنژیوژنزimmunoglobulin G - ایمونوگلوبولین GPositron emission tomography - توموگرافی گسیل پوزیترونGemcitabine - جمسیتابینfluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسPancreatic cancer - سرطان پانکراسfragment crystallizable - قطعه قابل برداشتنMatrix metalloproteinase-9 - ماتریکس متالوپروتئیناز -9Matrix metalloproteinase-2 - ماتریکس متالوپروتئیناز-2half maximal inhibitory concentration - نیمه حداکثر غلظت مهاریPET - پت
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Single domain antibody against carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) inhibits proliferation, migration, invasion and angiogenesis of pancreatic cancer cells Single domain antibody against carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) inhibits proliferation, migration, invasion and angiogenesis of pancreatic cancer cells](/preview/png/8443584.png)
چکیده انگلیسی
Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is over-expressed in pancreatic cancer cells, and it is associated with the progression of pancreatic cancer. We tested a single domain antibody (sdAb) targeting CEACAM6, 2A3, which was isolated previously from a llama immune library, and an Fc conjugated version of this sdAb, to determine how they affect the pancreatic cancer cell line BxPC3. We also compared the effects of the antibodies to gemcitabine. Gemcitabine and 2A3 slowed down cancer cell proliferation. However, only 2A3 retarded cancer cell invasion, angiogenesis within the cancer mass and BxPC3 cell MMP-9 activity, three features important for tumour growth and metastasis. The IC50s for 2A3, 2A3-Fc and gemcitabine were determined as 6.5 μM, 8 μM and 12 nM, respectively. While the 2A3 antibody inhibited MMP-9 activity by 33% compared to non-treated control cells, gemcitabine failed to inhibit MMP-9 activity. Moreover, 2A3 and 2A3-Fc inhibited invasion of BxPC3 by 73% compared to non-treated cells. When conditioned media that were produced using 2A3- or 2A3-Fc-treated BxPC3 cells were used in a capillary formation assay, the capillary length was reduced by 21% and 49%, respectively. Therefore 2A3 is an ideal candidate for treating tumours that over-express CEACAM6.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cancer - Volume 50, Issue 4, March 2014, Pages 713-721
Journal: European Journal of Cancer - Volume 50, Issue 4, March 2014, Pages 713-721
نویسندگان
Tsai-Mu Cheng, Yanal M. Murad, Chia-Ching Chang, Ming-Chi Yang, Toya Nath Baral, Aaron Cowan, Shin-Hua Tseng, Andrew Wong, Roger MacKenzie, Dar-Bin Shieh, Jianbing Zhang,