کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8455979 | 1548363 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Measurement of oxidatively generated base damage in cellular DNA
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کلمات کلیدی
5-Formyl-2′-deoxyuridineRNS8-oxodGuoDSBs8-oxoGuaFapyGuaFapyAde2,6-diamino-4-hydroxy-5-formamidopyrimidine - 2،6-دیامینو-4-هیدروکسی-5-فرمامیدوپیریمیدین8-oxo-7,8-dihydro-2′-deoxyguanosine - 8-اکسو-7،8-دی هیدرو-2'-دگزسی گوانوزین8-oxo-7,8-dihydroguanine - 8-اکسو-7،8-دی هیدروگوانینROS - ROSSSBs - SSBImmunoassays - آزمایشات ایمنیsingle strand breaks - شکاف تک رشته ایdouble strand breaks - شکست دو رشتهreactive nitrogen species - گونه های واکنش پذیر نیتروژنReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This survey focuses on the critical evaluation of the main methods that are currently available for monitoring single and complex oxidatively generated damage to cellular DNA. Among chromatographic methods, HPLC-ESI-MS/MS and to a lesser extent HPLC-ECD which is restricted to a few electroactive nucleobases and nucleosides are appropriate for measuring the formation of single and clustered DNA lesions. Such methods that require optimized protocols for DNA extraction and digestion are sensitive enough for measuring base lesions formed under conditions of severe oxidative stress including exposure to ionizing radiation, UVA light and high intensity UVC laser pulses. In contrast application of GC-MS and HPLC-MS methods that are subject to major drawbacks have been shown to lead to overestimated values of DNA damage. Enzymatic methods that are based on the use of DNA repair glycosylases in order to convert oxidized bases into strand breaks are suitable, even if they are far less specific than HPLC methods, to deal with low levels of single modifications. Several other methods including immunoassays and 32P-postlabeling methods that are still used suffer from drawbacks and therefore are not recommended. Another difficult topic is the measurement of oxidatively generated clustered DNA lesions that is currently achieved using enzymatic approaches and that would necessitate further investigations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 711, Issues 1â2, 3 June 2011, Pages 3-12
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 711, Issues 1â2, 3 June 2011, Pages 3-12
نویسندگان
Jean Cadet, Thierry Douki, Jean-Luc Ravanat,