کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456461 | 1548592 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A method for assessment of the genotoxicity of mainstream cigarette-smoke by use of the bacterial reverse-mutation assay and an aerosol-based exposure system
ترجمه فارسی عنوان
روشی برای ارزیابی ژنوتوکسیستی دود سیگار اصلی با استفاده از آزمون موتاژن معکوس باکتری و یک سیستم مواجهه مبتنی بر آئروسل
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کلمات کلیدی
PBSNQO2-aminoanthraceneNaN32NF2-nitrofluorene - 2-نیتروفلوئورنB[a]P - B [a] PDMSO - DMSOAAN - ONAmes - امزDose measurements - اندازه گیری دزISO - ایزوBenzo(a)pyrene - بنزو (a) پیرنهdimethyl sulphoxide - دی متیل سولفوکسیدsodium azide - سدیم آیزیدVapour phase - فاز بخارPhosphate buffered saline - فسفات بافر شورParticulate material - مواد جامدquartz crystal microbalance - میکرو ترازوی کریستال کوارتز، کوارتز کریستال میکرو بالانسQCM - میکروبازار کریستال کوارتز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
چکیده انگلیسی
To date there are no widely accepted methods for the toxicological testing of complex gaseous mixtures and aerosols, such as cigarette smoke, although some modifications to the standard regulatory methods have been developed and used. Historically, routine testing of cigarettes has primarily focused on the particulate fraction of cigarette smoke. However, this fraction may not accurately reflect the full toxicity and mutagenicity of the smoke aerosol as a whole, which contains semi-volatiles and short-lived products of combustion. In this study we have used a modified version of the bacterial reverse-mutation (Ames) assay for the testing of mainstream smoke generated from 3R4F reference cigarettes with a Vitrocell® VC 10 exposure system. This method has been evaluated in four strains of Salmonella typhimurium (TA98, TA100, YG1024 and YG1042) and one strain of Escherichia coli (WP2 uvrA pKM101) in the absence and presence of a metabolic activation system. Following exposure at four concentrations of diluted mainstream cigarette-smoke, concentration-related and reproducible increases in the number of revertants were observed in all four Salmonella strains. E. coli strain WP2 uvrA pKM101 was unresponsive at the four concentrations tested. To quantify the exposure dose and to enable biological response to be plotted as a function of deposited mass, quartz-crystal microbalances were included in situ in the smoke-exposure set-up. This methodology was further assessed by comparing the responses of strain YG1042 to mainstream cigarette-smoke on a second VC 10 Smoking Robot. In summary, the Ames assay can be successfully modified to assess the toxicological impact of mainstream cigarette-smoke.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 769, 15 July 2014, Pages 20-28
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 769, 15 July 2014, Pages 20-28
نویسندگان
Joanne Kilford, David Thorne, Rebecca Payne, Annette Dalrymple, Julie Clements, Clive Meredith, Debbie Dillon,