کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8461570 1549012 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The survival and proliferation of fibroblasts on biocomposites containing genetically modified flax fibers: An in vitro study
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
The survival and proliferation of fibroblasts on biocomposites containing genetically modified flax fibers: An in vitro study
چکیده انگلیسی
Natural fibers have long been used in several branches of industry. Nowadays, they are considered as composite materials in medicine with special focus on artificial tissue scaffolding, drug-release systems, cardiovascular patches and nerve cuffs. The purpose of this study has been to examine the in vitro biocompatibility of newly designed “green composites”. Therefore, composites containing flax fibers from transgenic flax plants producing polyhydroxybutyrate (M50) and control (wt-NIKE) plants in a polylactid (PLA) or polycaprolactone (PCL) matrix were prepared and mice fibroblast viability and cytotoxicity determined after incubation for 12-48 h and 3 weeks with those composites. After 24 h and 48 h, all green composites have a strong influence on cell viability and membrane stability without any differences among each other. The cell viability of treated cells is approximately 82.5-93% of those of untreated control cells, respectively. The increase in cytotoxicity ranged between 1.4 and 2.9 fold compared to untreated cells. After 3 weeks of incubation, no significant changes were detectable in the amount of dead and living cells between composite treated and untreated cells. In conclusion, the tested new “green composites” showed a good biocompatibility. The biocompatibility of composites from transgenic flax plant fibers producing PHB did not differ from composites of non-transgenic flax plant fibers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 194, Issue 6, November 2012, Pages 513-517
نویسندگان
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