Regional changes in the masseter muscle of rats after reduction of blood supply

A total of 20 adult male Wistar rats (Rattus norvegicus Berkenhout) were divided into an experimental group and a control group comprised of 10 animals respectively and kept under standardized conditions. The experimental group underwent a dextrolateral complete surgical ligation of the a. carotis communis and, after 5 weeks, specimens were taken from the masseters. The muscle samples were analyzed immunohistochemically for fiber distribution and capillary density. Analysis revealed a discrete increase in the proportion of type I fibers with a significant increase of capillary number per area. Although no agreement exists on the alterations occurring in chronically ischemic muscles, it may be assumed that chronic ischemia evokes histomorphological adaptation processes similar to endurance training effects.