A scanning electron microscopic study of nasal respiratory epithelium in pregnant rats

Pregnancy is a very important period in mammals. Various morphological and functional changes can be observed. Some studies examining nasal epithelial structure deal with changes found in pregnancy. In this study, a total of 16 adult female Sprague Dawley rats (8 control and 8 pregnant) were used. They were anesthetized with ketamine and sacrificed via cardiac perfusion with 2.5%-glutaraldehyde solution (in 0.1 M sodium phosphate buffer, pH 7.4). The heads of the rats were bisected midsagittally and one half was completely passed through the routine histological procedures for SEM examination. On the apical surfaces of ciliated cells of pregnant rat respiratory epithelium disoriented cilia and cilia with dilated blebs at their free ends were observed. In addition, some masses of mucus due to hypersecretion were striking. Goblet cells appeared swollen and their apical surfaces showed microvilli varying considerably in length and number.