کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8463702 | 1549394 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Droplet digital PCR quantifies host inflammatory transcripts in feces reliably and reproducibly
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Droplet digital PCR quantifies host inflammatory transcripts in feces reliably and reproducibly Droplet digital PCR quantifies host inflammatory transcripts in feces reliably and reproducibly](/preview/png/8463702.png)
چکیده انگلیسی
This method was applied to 799 fecal samples from rural Malawian children, and over 20,000 transcript concentrations were quantified. Host mRNA was detected in >99% samples, a threshold for target detection was established at an average expression of 0.02 copies target/GAPDH, above which correlation coefficient between duplicate measurements is >0.95. Quantities of transcript detected using ddPCR were greater than standard qPCR. Fecal sample preservation at the time of collection did not require immediate freezing or the addition of buffers or enzymes. Measurements of transcripts encoding immunoactive proteins correlated with a measure of gut inflammation in the study children, thereby substantiating their relevance. This method allows investigators to interrogate gene expression in the gut.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Immunology - Volume 303, May 2016, Pages 43-49
Journal: Cellular Immunology - Volume 303, May 2016, Pages 43-49
نویسندگان
Jennifer Stauber, Nurmohammad Shaikh, M Isabel Ordiz, Phillip I. Tarr, Mark J Manary,