Expression and characterization of recombinant soluble porcine CD3 ectodomain molecules: Mapping the epitope of an anti-porcine CD3 monoclonal antibody 898H2-6-15

► Porcine CD3 ectodomain molecules were expressed in Escherichia coli and refolded in vitro. ► The refolded porcine CD3 ectodomain molecules were purified with a strong anion exchange resin. ► Porcine CD3εγ ectodomain single-chain fusion protein can bind to an anti-porcine CD3 mAb 898H2-6-15.