Apocynin stimulates osteoblast differentiation and inhibits bone-resorbing mediators in MC3T3-E1 cells

► Apocynin increased alkaline phosphatase activity, collagen content, and mineralization in MC3T3-E1 cells. ► Apocynin increased cell survival, calcium deposition, and osteoprotegerin release. ► Apocynin decreased the production of ROS, TNF-α, IL-6, and RANKL in the presence of antimycin A.