کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8464112 1549437 2018 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The effect of isochoric freezing on mammalian cells in an extracellular phosphate buffered solution
ترجمه فارسی عنوان
اثر انجماد ایزووریک بر روی سلول های پستانداران در محلول بافر فسفات خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
Isochoric (constant volume) freezing has been recently suggested as a new method for cell and organ preservation. As a first step in studying the effect of isochoric freezing on mammalian cells, Madin-Darby canine kidney epithelial cells (MDCK), were frozen in an isochoric system, in a simple extracellular phosphate buffered solution to −10 °C (96.5 MPa), - 15 °C (162 MPa) and −20 °C (205 MPa) for 60 and 120 min. Cell membrane integrity and cell metabolism were studied with a Live/Dead cell vitality assay and flow cytometry. We found that cell survival decreases with an increase in pressure (lower temperatures) and time of exposure. For example, 60% of cells survived 60 min at - 10 °C and only 18% survived 120 min at this temperature. Negligible survival was measured at - 20 °C. This study may serve as the baseline towards further research on techniques to optimize the effects of isochoric freezing on living biological matter.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 82, June 2018, Pages 155-158
نویسندگان
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