کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8464353 | 1549439 | 2018 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study aims to assess the effect of Eurycoma longifolia aqueous extract on chilled and cryopreserved quality of bull sperm. Semen samples were obtained from four Simmental-Brangus. Each sample was divided into two fractions: the first fraction was used for chilling the semen, and the second fraction was used for the freezing process. Both fractions were extended with Tris-egg yolk extender supplemented with 0.0, 0.25, 0.5, 1.0, 2.5, 5.0, and 7.5â¯mg/ml Eurycoma longifolia aqueous extract. The diluted chilled fraction was chilled at 5â¯Â°C for 6 days, whereas the frozen-thawed fraction was frozen in liquid nitrogen. Data revealed that 1â¯mg/ml E. longifolia aqueous extract yielded significantly (pâ¯<â¯.05) higher sperm motility, morphology, viability, and sperm membrane integrity compared with the control group and other treated groups in chilled semen evaluation. For cryopreserved sperm, a significant difference (pâ¯<â¯.05) in sperm motility, viability, sperm membrane integrity, DNA integrity, and lipid peroxidation was observed between 5â¯mg/ml E. longifolia aqueous extract and other treated and control groups. However, no significant difference in the percentage of sperm exhibiting normal sperm morphology was observed among the groups. In conclusion, the addition of 0.25 and 1â¯mg/ml E. langifolia extract to chilled semen and 5â¯mg/ml E. longifolia aqueous extract to cryopreserved sperm into Tris-egg yolk extender helps in maintaining superior quality of bull spermatozoa during chilling and freezing.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 80, February 2018, Pages 43-50
Journal: Cryobiology - Volume 80, February 2018, Pages 43-50
نویسندگان
Falah H. Baiee, Haron Wahid, Yusoff Rosnina, Omar Ariff, Nurhusien Yimer, Zaid Jeber, Hammadi Salman, Ahmedeltayeb Tarig, Fazel Harighi,