Single-molecule imaging revealed dynamic GPCR dimerization

- Single-molecule imaging in live cells revealed GPCR dimer-monomer equilibrium.
- GPCRs continually form transient monomers and homo-dimers with different partners.
- GPCR homo-dimers dissociate into monomers on the time scale of 0.1 s at 37 °C.
- The monomer-dimer equilibrium was fully characterized by single-molecule imaging.
- Single-molecule imaging is powerful for elucidating signal transduction mechanisms.