The involvement of Eph-Ephrin signaling in tissue separation and convergence during Xenopus gastrulation movements

► We suggest Eph-ephrin signaling as a new major mechanism for tissue separation. ► EphA4 and ephrin-A1 are expressed in the mesoderm and ectoderm, respectively. ► Depletion of Eph-ephrin signaling perturbed tissue separation during gastrulation. ► Thus, Eph-ephrin signaling regulates tissue separation between mesoderm and ectoderm.