کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8468366 | 1549572 | 2010 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RPTPα and PTPε signaling via Fyn/Yes and RhoA is essential for zebrafish convergence and extension cell movements during gastrulation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
Convergence and extension (C&E) cell movements are essential to shape the body axis during vertebrate gastrulation. We have used the zebrafish to assess the role of the receptor protein-tyrosine phosphatases, RPTPα and PTPε, in gastrulation cell movements. Both RPTPα and PTPε knockdown and ptpraâ/â embryos show defects in C&E movements. A method was developed to track gastrulation cell movements using confocal microscopy in a quantitative manner and ptpraâ/â embryos displayed reduced convergence as well as extension speeds. RPTPα and PTPε knockdowns cooperated with knockdown of a well known factor in C&E cell movement, non-canonical Wnt11. RPTPα and PTPε dephosphorylate and activate Src family kinases in various cell types in vitro and in vivo. We found that Src family kinase phosphorylation was enhanced in ptpraâ/â embryos, consistent with reduced Src family kinase activity. Importantly, both ptpraâ/â and RPTPα and PTPε knockdown induced C&E defects were rescued by active Fyn and Yes. Moreover, active RhoA rescued the RPTPα and PTPε knockdown and ptpraâ/â induced gastrulation cell movement defects as well. Our results demonstrate that RPTPα and PTPε are essential for C&E movements in a signaling pathway parallel to non-canonical Wnts and upstream of Fyn, Yes and RhoA.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 340, Issue 2, 15 April 2010, Pages 626-639
Journal: Developmental Biology - Volume 340, Issue 2, 15 April 2010, Pages 626-639
نویسندگان
Mark van Eekelen, Vincent Runtuwene, John Overvoorde, Jeroen den Hertog,