کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8469791 | 1549674 | 2016 | 16 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gelsolin interacts with LamR, hnRNP U, nestin, Arp3 and β-tubulin in human melanoma cells as revealed by immunoprecipitation and mass spectrometry
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کلمات کلیدی
LAMrheterogeneous nuclear ribonucleoprotein UhnRNP UIgGshemagglutinin tagArp3RPSAGSNG-actinWCLPLAPICF-actinfilamentous actin - actin filamentousLC/MS - LC / MSProximity ligation assay - آزمون لمس نزدیکیMonomeric actin - آکنه مونومرimmunoglobulins - ایمونوگلوبولین هاImmunoprecipitation - تخریب ایمنیRoom temperature - دمای اتاقCo-IP - شرکت-IPwhole cell lysate - لسیات کل سلولnestin - نستینWestern blot - وسترن بلاتGelsolin - ژلزولینLiquid chromatography/mass spectrometry - کروماتوگرافی مایع / طیف سنج جرمیCytosolic fraction - کریستال سیتوزولNuclear fraction - کسری هسته ایprotease inhibitor cocktail - کوکتل مهار کننده پروتئازCommercial kit - کیت بازرگانیlaminin receptor - گیرنده لامینین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Gelsolin, a multifunctional actin binding protein, plays a not yet fully understood role in tumorigenesis. Therefore the goal of this study was to identify additional molecular partners of gelsolin in human melanoma cells, separately in the cytoplasmic compartment and cell nuclei. For this purpose we performed immunoprecipitation experiments based on a modified protocol followed by mass spectrometry. The obtained results were confirmed by Western blot analysis, proximity ligation assays and confocal microscopy. As expected gelsolin interacted with actin, in particular we demonstrate its interaction with cytoplasmic β and γ actins, and a newly discovered actin isoform, actbl2. As new gelsolin-interacting partners we identified the ribosomal protein Rpsa, also known as a non-integrin laminin receptor (LamR), and the heterogeneous nuclear ribonucleoprotein hnRNP U. Our data furthermore indicate that gelsolin interacts with particular components of the three cytoskeleton systems: nestin (intermediate filaments), Arp3 (actin cytoskeleton) and β-tubulin (microtubules). We also report for the first time that gelsolin is a constituent of midbodies, a tubulin containing structure formed at the end of cytokinesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 95, Issue 1, January 2016, Pages 26-41
Journal: European Journal of Cell Biology - Volume 95, Issue 1, January 2016, Pages 26-41
نویسندگان
Antonina Joanna Mazur, Tomasz Radaszkiewicz, Aleksandra Makowiecka, Maria Malicka-BÅaszkiewicz, Hans Georg Mannherz, Dorota Nowak,