کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8469967 1549687 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
JMY is involved in anterograde vesicle trafficking from the trans-Golgi network
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
JMY is involved in anterograde vesicle trafficking from the trans-Golgi network
چکیده انگلیسی
To address the precise cellular function of JMY in cellular actin rearrangements, we have searched for potential new interaction partners by mass spectrometry. We identified several candidates and correlated their localization with the subcellular dynamics of JMY. JMY is localized to dynamic vesiculo-tubular structures throughout the cytoplasm, which are decorated with actin and Arp2/3 complex. Moreover, JMY partially colocalizes and interacts with VAP-A, which is involved in vesicle-based transport processes. Finally, overexpression of JMY results in Golgi dispersal by loss from the trans-site and affects VSV-G transport. These analyses, together with biochemical experiments, indicate that JMY drives vesicular trafficking in the trans-Golgi region and at ER-membrane contact sites (MCS), distinct from other Arp2/3 activators involved in vesicle transport processes such as the related WHAMM or WASH.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 93, Issues 5–6, May–June 2014, Pages 194-204
نویسندگان
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