کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8470094 1549722 2010 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The sarcomeric Z-disc component myopodin is a multiadapter protein that interacts with filamin and α-actinin
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
The sarcomeric Z-disc component myopodin is a multiadapter protein that interacts with filamin and α-actinin
چکیده انگلیسی
Here we introduce myopodin as a novel filamin C binding partner. Corroborative yeast two-hybrid and biochemical analyses indicate that the central part of myopodin that shows high homology to the closely related protein synaptopodin and that is common to all its currently known or predicted variants interacts with filamin C immunoglobulin-like domains 20-21. A detailed characterization of the previously described interaction between myopodin and α-actinin demonstrates for the first time that myopodin contains three independent α-actinin-binding sites. Newly developed myopodin-specific antibodies reveal expression at the earliest stages of in vitro differentiation of human skeletal muscle cells preceding the expression of sarcomeric α-actinin. Myopodin colocalizes with filamin and α-actinin during all stages of muscle development. By contrast, colocalization with its previously identified binding partner zyxin is restricted to early developmental stages. Genetic and cellular analyses of skeletal muscle provided direct evidence for an alternative transcriptional start site in exon three, corroborating the expression of a myopodin variant lacking the PDZ domain encoded by exons 1 and 2 in skeletal muscle. We conclude that myopodin is a multiadapter protein of the sarcomeric Z-disc that links nascent myofibrils to the sarcolemma via zyxin, and might play a role in early assembly and stabilization of the Z-disc. Mutations in FLNC, ACTN2 and several other genes encoding Z-disc-related proteins cause myopathy and cardiomyopathy. Its localization and its association with the myopathy-associated proteins filamin C and α-actinin make myopodin an interesting candidate for a muscle disease gene.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Cell Biology - Volume 89, Issue 9, September 2010, Pages 681-692
نویسندگان
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