کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8470620 | 1550009 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The gene ICS3 from the yeast Saccharomyces cerevisiae is involved in copper homeostasis dependent on extracellular pH
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کلمات کلیدی
ICSCCC2CCS1Mac1ACE1FETMTSYPdCCOCTRFRE - آزادیCopper accumulation - انباشت مسCopper transporter - حمل و نقل مسSOD - سدcopper–zinc superoxide dismutase - سوپر اکسید دیسموتاز مس-رویcytochrome c oxidase - سیتوکروم سی اکسیدازAcidic conditions - شرایط اسیدیyeast extract peptone dextrose - عصاره مخمر پپتون دگزستروزMetallothioneins - متالوتیژیونCopper homeostasis - هومئوستاز مس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
In the yeast Saccharomyces cerevisiae, many genes are involved in the uptake, transport, storage and detoxification of copper. Large scale studies have noted that deletion of the gene ICS3 increases sensitivity to copper, Sortin 2 and acid exposure. Here, we report a study on the Îics3 strain, in which ICS3 is related to copper homeostasis, affecting the intracellular accumulation of this metal. This strain is sensitive to hydrogen peroxide and copper exposure, but not to other tested transition metals. At pH 6.0, the Îics3 strain accumulates a larger amount of intracellular copper than the wild-type strain, explaining the sensitivity to oxidants in this condition. Unexpectedly, sensitivity to copper exposure only occurs in acidic conditions. This can be explained by the fact that the exposure of Îics3 cells to high copper concentrations at pH 4.0 results in over-accumulation of copper and iron. Moreover, the expression of ICS3 increases in acidic pH, and this is correlated with CCC2 gene expression, since both genes are regulated by Rim101 from the pH regulon. CCC2 is also upregulated in Îics3 in acidic pH. Together, these data indicate that ICS3 is involved in copper homeostasis and is dependent on extracellular pH.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 82, September 2015, Pages 43-50
Journal: Fungal Genetics and Biology - Volume 82, September 2015, Pages 43-50
نویسندگان
C.A. Alesso, K.F. Discola, G. Monteiro,