کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8470760 1550012 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Flexible gateway constructs for functional analyses of genes in plant pathogenic fungi
ترجمه فارسی عنوان
دروازه انعطاف پذیر برای تجزیه و تحلیل عملکردی ژن ها در قارچ های بیماریزای گیاهی طراحی شده است
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
چکیده انگلیسی
Genetic manipulation of fungi requires quick, low-cost, efficient, high-throughput and molecular tools. In this paper, we report 22 entry constructs as new molecular tools based on the Gateway technology facilitating rapid construction of binary vectors that can be used for functional analysis of genes in fungi. The entry vectors for single, double or triple gene-deletion mutants were developed using hygromycin, geneticin and nourseothricin resistance genes as selection markers. Furthermore, entry vectors containing green fluorescent (GFP) or red fluorescent (RFP) in combination with hygromycin, geneticin or nourseothricin selection markers were generated. The latter vectors provide the possibility of gene deletion and simultaneous labelling of the fungal transformants with GFP or RFP reporter genes. The applicability of a number of entry vectors was validated in Zymoseptoria tritici, an important fungal wheat pathogen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 79, June 2015, Pages 186-192
نویسندگان
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