Évaluation du kit Diasorin Liaison® dsDNA pour la détection des anticorps anti-DNA natif

Screening and quantification of anti dsDNA antibodies are useful for positive diagnosis and follow-up of Systemic Lupus Erythematosus (SLE). Various assays exist based on different techniques which can influence the results. This study evaluated the specificity and the sentitivity of the Liaison® dsDNA Diasorin assay for the detection of anti dsDNA antibodies. We compared the results with those obtained with ELIA® dsDNA Pharmacia routinely used in our laboratory. The study has involved the sera of 153 systemic lupus erythematosus and 78 controls. The control group included 54 other autoimmune diseases and 24 patients without autoimmune markers. Liaison® dsDNA (Pharmacia) assay offers a sensitivity of 91,5% and a specificity > 99%. Correlation with the ELIA® dsDNA assay is 0,68. The automated Liaison® dsDNA Diasorin assay demonstrates its value for the detection and quantification of anti dsDNA antibodies in SLE patients.