Canonical Wnt signaling in the visceral muscle is required for left-right asymmetric development of the Drosophila midgut

► Canonical Wnt signaling is required for the LR asymmetric development of the midgut. ► Wnt signaling needs to be activated in the visceral muscle for normal gut laterality. ► Wnt signaling in the gut is activated with bilateral LR symmetry. ► Roles of Wnt signaling in LR asymmetric development may be conserved evolutionarily.