کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8477055 | 1550876 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Epigenetic mechanisms of melatonin action in human SH-SY5Y neuroblastoma cells
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کلمات کلیدی
CREBPI3KRORERKP300MAPK - MAPKAkt - آکتEpigenetic - اپی ژنتیکphospho-Akt - فسفات آکتphosphoinositide 3-kinase - فسفینوزیتید 3-کینازHistone hyperacetylation - هیستون هیپرساتیلینگcAMP response element binding protein - پروتئین اتصال دهنده عنصر پاسخ cAMPprotein kinase B - پروتئین کیناز Bmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استextracellular-regulated kinase - کیناز تنظیم شده خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have shown that melatonin induces histone hyperacetylation in vitro and in vivo. To clarify the mechanisms involved, we have now investigated its effects on histone acetylation and signaling pathways in human SH-SY5Y neuroblastoma cells, which express melatonin MT1 receptors. Melatonin caused significant concentration-dependent increases in both histone H3 and H4 acetylation. Blockade of melatonin receptors with luzindole abolished the histone hyperacetylating effect of melatonin, whereas inhibition of MAPK-ERK by PD98059 attenuated but did not block this effect. Melatonin treatment for 24-h decreased the levels of phospho-ERK1/2, but significantly increased Akt phosphorylation and protein expression of the histone acetyltransferase, p300. These findings suggest that the epigenetic effects of melatonin in SH-SY5Y cells are mediated by G protein-coupled MT1 melatonin receptors. Furthermore, upregulation of the histone acetyltransferase/transcriptional co-activator p300, along with phosphorylation of Akt, which is essential for p300 activation, appear to be key mechanisms underlying the epigenetic effects of melatonin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 402, 15 February 2015, Pages 57-63
Journal: Molecular and Cellular Endocrinology - Volume 402, 15 February 2015, Pages 57-63
نویسندگان
Yi Pan, Lennard P. Niles,