Unraveling the mode of action of an obesogen: Mechanistic analysis of the model obesogen tributyltin in the 3T3-L1 cell line

► We have confirmed the obesogenic properties of TBT using the Nile Red fluorescent assay. ► Use of Nile Red fluorescent assay has high potential for a first screening of potential obesogens. ► We contributed to further unravelling of the TBT-induced PPARγ signalling pathway. ► A lipid staining technique combined with transcriptomics for obesogenicity screening.