Cell biology of H2O2 generation in the thyroid: Investigation of the control of dual oxidases (DUOX) activity in intact ex vivo thyroid tissue and cell lines

► MβCD, depletor of cholesterol, activates DUOXes only in differentiated thyroid cells. ► Reversible tools to activate (NaF) and inhibit (22 °C) Gq-PLC cascade in intact cells. ► Arachidonate as a direct activator of DUOX1. ► New models of thyroid cell biology of H2O2 generation.