کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8477894 | 1550934 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Contributions of steroidogenic factor 1 to the transcription landscape of Y1 mouse adrenocortical tumor cells
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Contributions of steroidogenic factor 1 to the transcription landscape of Y1 mouse adrenocortical tumor cells Contributions of steroidogenic factor 1 to the transcription landscape of Y1 mouse adrenocortical tumor cells](/preview/png/8477894.png)
چکیده انگلیسی
The contribution of steroidogenic factor 1 (SF-1) to the gene expression profile of Y1 mouse adrenocortical cells was evaluated using short hairpin RNAs to knockdown SF-1. The reduced level of SF-1 RNA was associated with global changes that affected the accumulation of more than 2000 transcripts. Among the down-regulated transcripts were several with functions in steroidogenesis that were affected to different degrees-i.e., Mc2r > Scarb1 > Star â¥Â Hsd3b1 > Cyp11b1. For Star and Cyp11b1, the different levels of expression correlated with the amount of residual SF-1 bound to the proximal promoter regions. The knockdown of SF-1 did not affect the accumulation of Cyp11a1 transcripts even though the amount of SF-1 bound to the proximal promoter of the gene was reduced to background levels. Our results indicate that transcripts with functions in steroidogenesis vary in their dependence on SF-1 for constitutive expression. On a more global scale, SF-1 knockdown affects the accumulation of a large number of transcripts, most of which are not recognizably involved in steroid hormone biosynthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 336, Issues 1â2, 10 April 2011, Pages 85-91
Journal: Molecular and Cellular Endocrinology - Volume 336, Issues 1â2, 10 April 2011, Pages 85-91
نویسندگان
Bernard P. Schimmer, Jennivine Tsao, Martha Cordova, Sara Mostafavi, Quaid Morris, Joshua O. Scheys,