کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8478030 | 1550936 | 2011 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional expression of angiotensinogen depends on splicing enhancers in exon 2
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
Angiotensinogen belongs to the family of serpins and is the only precursor of the potent cardiovascular peptide, angiotensin II, the main effector of the renin-angiotensin system. The gene coding for this protein carries an internal exon (exon 2), the length of which (859Â bp) by far exceeds the mean length of internal exons in vertebrates (<300Â bp). Here, we show that this essential exon is skipped in about 20% of all transcripts in liver, brain, and kidney of rats and mice. Deletion mutants of exon 2 revealed a 62Â bp region located at its 5â²-end which is important for its inclusion in the mature angiotensinogen mRNA in transfected COS7 cells. Using an artificial minigene, we defined sequences inside this region as exonic splicing enhancers. These data reveal a novel molecular mechanism important for the renin-angiotensin system with implications in the basic understanding and the therapeutical assessment of cardiovascular diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 332, Issues 1â2, 30 January 2011, Pages 228-233
Journal: Molecular and Cellular Endocrinology - Volume 332, Issues 1â2, 30 January 2011, Pages 228-233
نویسندگان
Cibele C. Cardoso, Daniela A. Cabrini, Markus May, Claudia S. Bhagat, Nelida Eleno, Cécile Cayla, Thomas Walther, Michael Bader,